Plant Tissue Culture Ppt Pdf đ„
Plant cells are treated enzymatically to remove their cell walls. The resulting naked cells (protoplasts) can be fused to create somatic hybrids between different species. 4. The 5 Stages of Micropropagation
The LMS.SU.EDU.PK platform offers a downloadable media-preparation.pdf file that walks readers through the entire process. The NPTEL archive provides an excellent lecture on tissue culture media that includes step-by-step preparation instructions. plant tissue culture ppt pdf
, the ability of a single plant cell to regenerate into a whole new plant. Core Techniques & Types Different methods are used depending on the target tissue: Callus Culture: Growing unorganised masses of cells. Organ Culture: Focused on specific parts like embryos, seeds, or ovaries. Meristem Culture: Used to produce virus-free plants from the shoot tip. Protoplast Fusion: Fusing cells without walls to create hybrids. Anther/Pollen Culture: Developing haploid plants. TNAU Agritech The 5 Main Stages of Culture Stage 0: Selection & Preparation: Choosing a healthy "explant" (source plant part). Stage 1: Initiation/Establishment: Sterilising the explant and placing it on a growth medium. Stage 2: Multiplication: Inducing the tissue to produce multiple shoots or embryos. Stage 3: Rooting: Plant cells are treated enzymatically to remove their